James   Bullard

Associate Professor  

Office: ESCNE 3.320
Phone: (956) 665-2950
Email: james.bullard@utrgv.edu

Dr. James Bullard

  • Areas of Expertise

    • My research involves development of protein synthesis systems from Pseudomonas aeruginosa for use as screening platforms for identification of compounds that inhibit enzymatic activity and bacterial growth. Proteins involved in protein synthesis are cloned, purified, and characterized. Ribosomes are isolated from large growths of P. aeruginosa. Platforms for screening compound libraries are developed from the total protein synthesis machinery and from individual components involved in protein synthesis. Hit compounds coming out of screens are analyzed for inhibition of enzymatic activity, mode of activity as well as anti-bacterial activity. Interesting compounds are tested against eukaryotic protein synthesis systems as well as against human cell cultures. Compounds are further advanced by structure activity relationship (SAR) analysis. SAR driven derivatives of hit compounds are designed, synthesized and analyzed in enzymatic assays and against bacterial growth. From all this, lead compound series are developed and further analyzed against human cell components such as various receptors to detect possible toxicity. We also generate bacteria with resistance to the lead compounds and use the crystal structure of protein targets to model the compound binding as well as the target mutations we have identified to understand how resistance is developed. Initial ADME studies are undertaken to determine bioavailability and elimination of lead compounds.
  • Education

    • BS, Biology, Southeastern Oklahoma State University.
    • PhD, Microbiology, The University of Montana, 1996
  • Currently Teaching

    UNIV 4000 08 - Directed Research:
    Syllabus
    Course Evaluations
    Textbooks




Selected Publications


  • Y Hu, S Palmer, T Resto, Frank Dean, and James Bullard. "Identification of Chemical Compounds That Inhibit the Function of Histidyl-tRNA Synthetase from Pseudomonas aeruginosa.," SLAS discovery 23, (January (1st Quarter/Winter) 2018): 65-75.

  • S Robles, Y Hu, T Resto, Frank Dean, and James Bullard. "Identification and Characterization of a Chemical Compound that Inhibits Methionyl-tRNA Synthetase from Pseudomonas aeruginosa.," Current drug discovery technologies 14, (July (3rd Quarter/Summer) 2017): 156-168.

  • Y Hu, A Bernal, James Bullard, and Yonghong Zhang. "Solution structure of protein synthesis initiation factor 1 from Pseudomonas aeruginosa.," Protein science : a publication of the Protein Society 25, no. 12 (December 2016): 2290-2296.

  • S Palmer, Y Hu, Megan Keniry, and James Bullard. "Identification of Chemical Compounds That Inhibit Protein Synthesis in Pseudomonas aeruginosa.," Journal of biomolecular screening (November 2016).

  • Y Hu, Megan Keniry, S Palmer, and James Bullard. "Discovery and Analysis of Natural-Product Compounds Inhibiting Protein Synthesis in Pseudomonas aeruginosa.," Antimicrobial agents and chemotherapy 60, no. 8 (July (3rd Quarter/Summer) 2016): 4820-9.

  • A Bernal, Y Hu, S Palmer, Alfred Silva, James Bullard, and Yonghong Zhang. "1H, 13C and 15N resonance assignments and secondary structure analysis of translation initiation factor 1 from Pseudomonas aeruginosa.," Biomolecular NMR assignments (March 2016).

  • Y Hu, E Guerrero, Megan Keniry, J Manrrique, and James Bullard. "Identification of Chemical Compounds That Inhibit the Function of Glutamyl-tRNA Synthetase from Pseudomonas aeruginosa.," Journal of biomolecular screening 20, no. 9 (October (4th Quarter/Autumn) 2015): 1160-70.

  • W Ribble, S Kane, and James Bullard. "Long-Range PCR Amplification of DNA by DNA Polymerase III Holoenzyme from Thermus thermophilus.," Enzyme research 2015, (January (1st Quarter/Winter) 2015): 837842.

  • Y Hu, S Palmer, Hugo Munoz, and James Bullard. "High Throughput Screen Identifies Natural Product Inhibitor of Phenylalanyl-tRNA Synthetase from Pseudomonas aeruginosa and Streptococcus pneumoniae.," Current drug discovery technologies 11, no. 4 (January (1st Quarter/Winter) 2014): 279-92.

  • S Palmer, E Rangel, Y Hu, A Tran, and James Bullard. "Two homologous EF-G proteins from Pseudomonas aeruginosa exhibit distinct functions.," PloS one 8, no. 11 (November 2013): e80252.



Selected Grants and Fellowships


  • James Bullard. Construction of a Protein Synthesis System from Pseudomonas aeruginosa for Screening for Antibacterial Candidates, Department of Health and Human Services, National Institutes of Health, National Institute of General Medical Sciences , $435,900.00 (May 2016 - April 2020)

  • Yonghong Zhang. Bacterial Protein Synthesis as a Target for Novel Antibacterial Agents, The Office of Research, Innovation, & Economic Development, $20,000.00 (January 2017 - August 2018)

  • James Bullard. A Protein Synthesis System from Pseudomonas aeruginosa for Screening for Antibacterials, Department of Health and Human Services, National Institutes of Health, National Institute of General Medical Sciences , $430,800.00 (May 2012 - April 2016)

  • James Bullard. Development of a Hybrid Streptococcal Protein Synthesis Screening Platform, University of Texas System UT Transform, $10,000.00 (2013 - 2014)

  • James Bullard. Expression Optimization and Characterization of Protein Synthesis Elongation Factor G (EF-G) from Bacillus subtilis, University of Texas - Pan American URI, $2,000.00 (2012 - 2013)

  • James Bullard. Cloning and Transformation of AlaRS, ArgRS, AspRS and GluRS from Pseudomonas aeruginosa, Faculty Research Council Award – University of Texas- Pan American , $5,000.00 (2010 - 2011)

  • James Bullard. Development of a Minimum Bacterial Protein Synthesis System for Discovery of New Antibiotics Beneficial to South Texas Inhabitants, The South Texas Border Health Disparities Center Award, $15,000.00 (2010 - 2011)

  • James Bullard. Molecular Cloning of Leucyl-tRNA Synthetase from Pseudomonas aeruginosa, the most Significant Pathogen causing Hospital-acquired Infections, University of Texas - Pan American URI, $2,000.00 (2010 - 2011)

  • James Bullard. Cloning and Characterization of DnaE2 from P. aeruginosa, the most Significant Pathogen Causing Hospital Acquired Infections, University of Texas - Pan American FRC, $5,000.00 (2009 - 2010)

  • James Bullard. Development of a Minimum Bacterial Protein Synthesis System for Discovery of New Antibiotics Beneficial to South Texas Inhabitants, The South Texas Border Health Disparities Center Award, $15,000.00 (2009 - 2010)



Selected Presentations


  • James Bullard and Casey Hughes. "Development of a Natural Protein Synthesis System from Pseudomonas aeruginosa," American Society for Microbiology/Interscience Conference on Antimicrobial Agents and Chemtherapy, New Orleans, LA (June 2017)

  • James Bullard and Samantha Balboa. "Development of Lysyl-tRNA Synthetase from Pseudomonas aeruginosa as a Platform to Screen for Inhibitors of Protein Synthesis," American Society for Microbiology/Interscience Conference on Antimicrobial Agents and Chemtherapy, New Orleans, LA (June 2017)

  • James Bullard and Yaritza Escamilla. "Identification of Compounds that Inhibit the Function of GlnRS from Pseudomonas aeruginosa," American Society for Microbiology/Interscience Conference on Antimicrobial Agents and Chemtherapy, New Orleans, LA (June 2017)

  • James Bullard and Noah Pena. "Cloning and Screening of Prolyl-tRNA Synthetase from Pseudomonas aeruginosa to Identify Protein Synthesis Inhibitors," American Society for Biochemistry and Molecular Biology, San Diego, CA (April 2016)

  • James Bullard and Casey Hughes. "Development of a Natural Protein Synthesis System from Pseudomonas aeruginosa," American Society for Biochemistry and Molecular Biology, San Diego, CA (April 2016)

  • James Bullard and Daniel Cantu. "Development of Arginyl-tRNA Synthetase from Pseudomonas aeruginosa as a Platform to Screen for Inhibitors of Protein Synthesis," American Society for Biochemistry and Molecular Biology, San Diego, CA (April 2016)

  • James Bullard and Yaritza Escamilla. "Development of Glutaminyl-tRNA Synthetase from Pseudomonas aeruginosa as a Platform to Screen for Inhibitors of Protein Synthesis," American Society for Biochemistry and Molecular Biology, San Diego, CA (April 2016)

  • James Bullard and Sara Robles. "Development of Methionyl-tRNA Synthetase from Pseudomonas aeruginosa as a Platform to Screen for Inhibitors of Protein Synthesis," American Society for Biochemistry and Molecular Biology, San Diego, CA (April 2016)

  • James Bullard and Stephanie Palmer. "A Screening Platform for Identification of Compounds that Inhibit Protein Synthesis in Pseudomonas aeruginosa," American Society for Microbiology, New Orleans, LA (June 2015)

  • James Bullard and Regina Zamacona. "Discovery of Chemical Compounds that Inhibit the Activity of Leucyl-tRNA Synthetase from Pseudomonas aeruginosa," American Society for Microbiology, New Orleans, LA (June 2015)

Current Courses

UNIV 4000 08 - Directed Research:
Syllabus
Course Evaluations
Textbooks

Previous Courses