Frank   B.  Dean

Assistant Professor  

Office: ESCNE 3.368
Phone: (956) 665-2852
Email: frank.dean@utrgv.edu

Frank B. Dean

  • Areas of Expertise

    • Endocrine Disrupting Chemicals (EDCs) are chemical contaminants in the environment that cause birth and developmental defects in young children and in wildlife. The UN has identified EDCs as a serious problem worldwide. Yet the mechanism of action of EDCs is poorly understood. Dr. Dean’s research investigates the regulation and/or inhibition of estrogen receptor and androgen receptor by thyroid hormone, DDT, BPA, and other diphenyl compounds. His students have shown that EDCs such as DDT and BPA act by binding to the BF-3 site on these receptors. The research goal is to describe the mechanism of action of these EDCs at the BF-3 site and the techniques used include in vitro steroid binding assays and gene expression studies in tissue culture cells. One goal is to address the relationship between the mechanism of toxicity at low concentrations of chemical, over long years of exposure in the environment, and the mechanism of effects seen in the lab at higher concentrations of chemical over shorter incubation times. This work may lead to a streamlined assay for endocrine disrupting activity for chemical testing. A long-term goal is the discovery of new compounds that bind to BF-3 but that could reverse the toxic effects of EDCs in the body. In addition, validation of BF-3 as a target for binding of allosteric regulatory compounds may lead to new screens for compounds against breast cancer and prostate cancer.
    • Dr. Dean’s early research specialized in the study of DNA replication. For his graduate work he determined that the mechanism of E. coli DNA topoisomerase I was via a single-strand DNA strand passage process. For his postdoctoral work he described the mechanism of SV40 viral DNA replication initiation and DNA unwinding by the SV40 T antigen. He then uncovered and cloned the genes for one of the human DNA repair/cell cycle checkpoint pathways. He worked at a biotech start-up company until 2002, where he developed a method for 100,000-fold amplification of plasmid and genomic DNA, which is now a widely used technique, whole genome amplification (WGA). He then worked at a second biotech company, until 2008, where he gained experience in drug discovery of new antibiotics. He came to UTPA in 2009 and joined UTRGV in 2015. He has authored or co-authored 46 scientific articles, and eight U.S. patents. ResearchGate reports that his publications have received 6,360 total citations in the literature.
  • Education

    • PhD, Biochemistry, University of Chicago, 1984
    • BA, Biochemical Sciences, Harvard University, 1978
  • Currently Teaching

    UNIV 4000 06 - Directed Research:
    Syllabus
    Course Evaluations
    Textbooks
    CHEM 1312 13P - General Chemistry II:
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    Course Evaluations
    Textbooks
    CHEM 1312 13P - General Chemistry II:
    Syllabus
    Course Evaluations
    Textbooks
    CHEM 3103 05 - Biochemistry I Lab:
    Syllabus
    Course Evaluations
    Textbooks
    CHEM 1312 09P - General Chemistry II:
    Syllabus
    Course Evaluations
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    CHEM 1312 09P - General Chemistry II:
    Syllabus
    Course Evaluations
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    CHEM 3303 02 - Biochemistry I:
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    Course Evaluations
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Selected Publications


  • Y Hu, S Palmer, T Resto, Frank Dean, and James Bullard. "Identification of Chemical Compounds That Inhibit the Function of Histidyl-tRNA Synthetase from Pseudomonas aeruginosa.," SLAS discovery 23, (January (1st Quarter/Winter) 2018): 65-75.

  • S Robles, Y Hu, T Resto, Frank Dean, and James Bullard. "Identification and Characterization of a Chemical Compound that Inhibits Methionyl-tRNA Synthetase from Pseudomonas aeruginosa.," Current drug discovery technologies 14, (July (3rd Quarter/Summer) 2017): 156-168.

  • J Guiles, X Sun, I Critchley, U Ochsner, M Tregay, K Stone, J Bertino, L Green, R Sabin, Frank Dean, H Dallmann, C McHenry, and N Janjic. "Quinazolin-2-ylamino-quinazolin-4-ols as novel non-nucleoside inhibitors of bacterial DNA polymerase III," Bioorg Med Chem Lett 19, (2009): 800-802.

  • L Green, James Bullard, W Ribble, Frank Dean, D Ayers, U Ochsner, N Janjic, and T Jarvis. "Inhibition of methionyl-tRNA synthetase by REP8839 and effects of resistance mutations on enzyme activity.," Antimicrobial agents and chemotherapy 53, no. 1 (January (1st Quarter/Winter) 2009): 86-94.

  • U Ochsner, C Young, K Stone, Frank Dean, N Janjic, and I Critchley. "Mode of Action and Biochemical Characterization of REP8839, a Novel Inhibitor of Methionyl-tRNA Synthetase," Antimicrob Agents Chemother 49, (2005): 4253-4262.

  • S Hosono, A Faruqi, Frank Dean, Y Du, Z Sun, X Wu, J Du, S Kingsmore, M Egholm, and R Lasken. "Unbiased whole-genome amplification directly from clinical samples," Genome Research 13, (2003): 954-964.

  • Frank Dean, S Hosono, L Fang, X Wu, A Faruqi, P Bray-Ward, Z Sun, Q Zong, Y Du, J Du, M Driscoll, W Song, S Kingsmore, M Egholm, and R Lasken. "Comprehensive Human Genome Amplification Using Multiple Displacement Amplification," Proc Natl Acad Sci USA 99, (2002): 5261-5266.

  • A Faruqi, S Hosono, M Driscoll, Frank Dean, O Alsmadi, R Bandaru, G Kumar, B Grimwade, Q Zong, Z Sun, Y Du, S Kingsmore, T Knott, and R Lasken. "High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplification," BMC Genomics 2, (2001): 4.

  • Frank Dean, J Nelson, T Giesler, and R Lasken. "Rapid amplification of plasmid and phage DNA using Phi 29 DNA polymerase and multiply-primed rolling circle amplification," Genome Research 11, (2001): 1095-1099.

  • T Eki, Frank Dean, A Kohda, K Okumura, M Abe, Y Murakami, M Ishiai, K Satomoto, J Hurwitz, M O'Donnell, and F Hanaoka. "Assignment of the homologue of the yeast origin recognition complex subunit ORC4 (ORC4L) to human chromosome band 2q22-->q23 by in situ hybridization and somatic cell hybrid analysis," Cytogenet Cell Genet 81, (1998): 89-90.



Selected Grants and Fellowships


  • Armin Solis. Engaged Scholarship Award, UTRGV, $2,000.00 (March 21, 2017 - August 31, 2017)

  • Ventura Flores. Louis Stokes Alliances for Minority Participation (LSAMP) , NSF, $4,800.00 (June 9, 2016 - August 15, 2016)



Selected Presentations


  • Frank Dean, Tahyra Resto, Amanda Chavez, and Ventura Flores. "The Effect of 4,4’ DDT, 4,4’ DDE, 4,4’ DDD, and 2,4’ DDD on Dihydrotestosterone Binding to and Releasing From the Androgen Receptor," SfE BES 2017, Society for Endocrinology, Harrogate, England, UK (November 2017)

  • Frank Dean, Shuchita Jhaveri, and Luis Perez. "Effects of diethylstilbestrol, tamoxifen, 4-hydroxytamoxifen, endoxifen, and resveratrol on steroid binding to the estrogen receptor," 2017 ACS Southwest Regional Meeting, American Chemical Society, Lubbock, TX (October 2017)

  • Evangelia Kotsikorou, Brenda Ortiz, and Frank Dean. "Molecular Modeling of the Estrogen Receptor Binding Function 3 Interactions with DiethylStilbestrol, Tamoxifen, and Its Analogues," 2017 ACS Southwest Regional Meeting, American Chemical Society, Lubbock, TX (October 2017)

  • Armin Solis and Frank Dean. "Regulation of estrogen binding to the estrogen receptor by endocrine disrupting chemicals," 2nd Annual Engaged Scholar Symposium , Office of Engaged Scholarship & Learning, UTRGV (April 2017)

  • Tahyra Resto, Frank Dean, Amanda Chavez, and Ventura Flores. "Effect of 4,4’ DDT, 4,4’ DDE, 4,4’ DDD, and 2,4’ DDD on Dihydrotestosterone binding to and releasing from the Androgen Receptor," 253rd American Chemical Society National Meeting,, American Chemical Society, San Francisco, CA (April 2017)

  • Tahyra Resto, Ventura Flores, Amanda Chavez, Monica Solis, and Frank Dean. "The Effect of 4,4’-DDT, 4,4’-DDE, 4,4’-DDD, 2,4’-DDD on Dihydrotestosterone Binding to the Androgen Receptor," UTSA College of Sciences Research Conference,, UTSA, San Antonio, TX (October 2016)

  • Ventura Flores and Frank Dean. "Regulation of Steroid Binding to the Androgen Receptor by DDT (dichlorodiphenyltrichloroethane)," 2016 University of Texas System Louis Stokes Alliance for Minority Participation (UT LSAMP) Student Research Conference,, UTEP, El Paso, TX (September 2016)

  • Antara Banik, Michael Mederos, James Bullard, and Frank Dean. "A Study Exploring the Inhibition of Dexamethasone Binding to the Glucocorticoid Receptor by the Thyroid Hormones and Their Analogues," HESTEC Science Symposium,, University of Texas-Pan American, Edinburg, TX (September 2011)

  • Michael Mederos, Antara Banik, James Bullard, and Frank Dean. "Allosteric Regulation of Glucocorticoid Receptor Ligand Binding by Fenamic and Arylalkanoic Acid Derivative NSAIDs," HESTEC Science Symposium,, University of Texas-Pan American, Edinburg, TX (September 2011)

  • Andrea Jimenez, Hazel Severino, Benxu Cheng, Frank Dean, and Fredrik Leeb-Lundberg. "Regulation of Breast Cancer Cell Growth by G Protein-coupled Estrogen Receptor 1 (GPER1)," HESTEC Science Symposium,, University of Texas-Pan American, Edinburg, TX (September 2011)

Current Courses

UNIV 4000 06 - Directed Research:
Syllabus
Course Evaluations
Textbooks
CHEM 1312 13P - General Chemistry II:
Syllabus
Course Evaluations
Textbooks
CHEM 1312 13P - General Chemistry II:
Syllabus
Course Evaluations
Textbooks
CHEM 3103 05 - Biochemistry I Lab:
Syllabus
Course Evaluations
Textbooks
CHEM 1312 09P - General Chemistry II:
Syllabus
Course Evaluations
Textbooks
CHEM 1312 09P - General Chemistry II:
Syllabus
Course Evaluations
Textbooks
CHEM 3303 02 - Biochemistry I:
Syllabus
Course Evaluations
Textbooks

Previous Courses